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I'm allways tweaking and improving my keys, brushes, efx stack bins, room layouts etc.. most of the time it's a progressive change caused by something and if lost couldn't just be recreated the same again so I'm backing up things all the time just in case but you know how it is, you'r way into something and you forget and then bam disaster strikes.

Is there some way to backup every little change you can make inside TVPaint in one fell swoop?

PC : You open "documents and settings" => User name" => "application data" => "TVP Animation 9 Pro" and you copy the "Default" folder =)

Mac : 'Library"=>"TVP Animation 9 Pro" => and you copy the "Default" folder =)

Great I'm allready doing that, I just backup the whole application data folder with SyncBackPro so I guess I'm covered

Was just a bit unsure if application data also contained my brushes

Yes, it contains everything.

But the folder isn't necessarily called "Default" if you have created new configs in the startup panel.
In this case there will be one folder per config.

No worries, I backup the whole application data folder as part of my normal backup routine so every programs configs stored in there will be backed up even.

I love how harddrives are so cheap now, I have 2 1TB drives I alternate my backups between. I use a program called SyncBackPro that's realy easy to do complicated backup schemes with. It syncronizes and even has revision control so you can tell it to keep say 7 revisions and backup each day. Then even if you make a mistake when saving you can roll back to the revision from the last day. Doesn't even take up that much more space as it only adds a revision for files that has changed.